Daya Antibakteri Infusa Annona muricata L. dalam Menghambat Bakteri Escherichia coli secara In Vitro

Abstract

Abstract

Gastrointestinal tract infections caused by Escherichia coli or diarrhea is one of the most common disease encountered in the community. The usual treatment for diarrhea is by taking antibiotics which can inhibit the growth or kill bacteria which cause diarrhea. However, antibiotics also have harmful effects to the body so that it needs another safe alternative treatment for the body. One of these alternative treatments is by ausing the leaves of the soursop (Annona muricata L.) were empirically containing antibacterial compounds such as alkaloids, tannins, flavonoids and saponins. This type of research is experimental. The sample was pure culture of E. coli. Dilution soursop leaves infuse include 25%, 50%, 75%, 100%. Antibacterial inhibition was obtained by measuring the inhibition zone formed around the paper dics. The data were analyzed descriptively. The results showed that the average diameter of inhibitory zone by Escherichia coliat for the concentration of soursop leaves infuse 25% (0 mm), 50% (4.2 mm), 75% (5.8 mm) and 100% (6.8 mm). Soursop leaf infusion can inhibit the growth of E. coli however it is still not effective to inhibit because the average diameter of inhibition zone formed relatively small at less than 10 mm.

Keywords: leaves of the soursop (Annona muricata L.), Escherichia coli, diarrhea

Abstrak

Infeksi saluran cerna disebabkan oleh Escherichia coli atau diare termasuk kejadian yang masih sering dijumpai di masyarakat. Langkah pengobatan untuk diare yaitu dengan pemberian antibiotik yang dapat menghambat pertumbuhan atau membunuh bakteri penyebab diare. Namun pemberian antibiotik juga memiliki dampak yang berbahaya bagi tubuh sehingga dibutuhkan alternatif pengobatan lain yang aman bagi tubuh. Salah satunya alternatif pengobatan dengan menggunakan daun sirsak (Annona muricata L.) yang secara empiris mengandung senyawa antibakteri seperti alkaloid, tanin, flavonoid dan saponin. Jenis penelitian adalah experimental. Sampel penelitian ini adalah kultur murni E.coli. Pengenceran infusa daun sirsak antara lain 25%, 50%, 75%,100%. Daya hambat  antibakeri diperoleh berdasarkan pengukuran zona hambat yang terbentuk disekitar paper dics. Analisis data dilakukan secara deskriptif. Hasil penelitian menunjukkan bahwa rata-rata diameter zona hambat oleh E.coli  pada konsentrasi infusa daun sirsak 25% (0 mm), 50 % (4,2 mm), 75% ( 5,8 mm) dan 100 % (6,8 mm). Infusa daun sirsak dapat menghambat pertumbuhan E.coli namun masih belum efektif untuk menghambat karena rata-rata diameter zona hambat yang terbentuk relatif kecil yaitu dibawah 10 mm.

Kata kunci : Daun sirsak (Annona muricata L.), Escherichia coli, Diare.