Overview of Giemsa, Wright and Wright-Giemsa Staining on Peripheral Blood Smear

Abstract

One type of complete blood count test is differential leukocyte count. this test uses peripheral blood smear. There are 3 staining methods for Peripheral Blood Smear (PBS), those are giemsa, wright, and wright-giemsa.

This type of research is a descriptive study. The test material used in this study was 3 ml of EDTA blood, which was later made into 30 slides of peripheral blood smear. The 30 slides will be population as well as the sample. The variables of this study were single variables.

From this study, the results obtained are that the best stain that can be used to observe erythrocyte cells is the wright-giemsa stain. For the leukocyte nuclei, we can be use wright stain, or a combination of wright and giemsa. For basophil granules, a combination of wright and giemsa can be used. For eosinopil cell’s granules can be observed using wright stain. The graules of neutrophil can be observed using giemsa stain. The cytoplasm of lymphocytes can be stained by giemsa or a combination of wright-giemsa. To observe the cytoplasm of monocytes, wright stain can be used. And for the observation of platelets, preparations can be stained using giemsa or wright stains.